ID: |
TKG 0175 |
Cell name: |
P3X63Ag8.653 (CRL1580) |
Data accepted: |
Dec.13, 1979 |
Animal: |
Mouse (BALB/c). |
Scientific name: |
Mus musculus. |
Sex: |
|
Case history: |
|
Tissue: |
Myeloma (non-secreting myeloma) |
Gentics: |
|
Life span: |
Infinite. |
Morphology: |
Myeloma-like
|
Medium: |
GIT, or RPMI-1640 + 10% FBS.
|
Passage method: |
Dilute 1/8 every 3-4 days. |
Characteristics: |
Mouse B cell hybridoma parther, HGPRT(-). This myeloma line is compatible to
other myeloma lines used in fusion process with respect to growth, fusion
frequency and stability.*
|
Temperature: |
|
CO2 concentration: |
|
Subculture frequency: |
|
Classification: |
|
Mycoplasma: |
|
Isozyme analysis: |
|
Established by: |
Kearney et al. |
References: |
J. Immunol., 123, 1548-1551, 1979. J. Immunol., 143, 2273-2279, 1979. Tohoku
J. Exp. Med., 153, 55-66, 1987. *Best fusion frequency can be obtained by
cultivation with GIT culuture medium.
|
References by users: |
|
Comments: |
|
Deposited by: |
Kudo, T.
|
Restriction: |
|
Photo: |
|