TKG 0185 ：： AZ521
|Scientific name：||Homo sapiens.|
|Medium：||DMEM + 10% MEM + 10% FBS + NEAA.|
|Passage method：||0.02% EDTA-PBS or 0.25% trypsin.|
|Characteristics：||Gastric cancer. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.|
|References：||Br. J. Cancer, 59, 761-765, 1989. PMID: 2736210|
|References by users：|
|Comments：||AZ-521 cell line was reported to be established from human gastric carcinoma
in Japan. However, the STR DNA analysis pattern of AZ-521 revealed to be
identical to that of other cell line derived from human duodenum carcinoma,
HuTu 80 in ATCC (registry number, HTB-40). This was confirmed by the Sanger
Institute's analysis of identities of human cancer cell lines. http://www.sanger.ac.uk/genetics/CGP/CellLines/
Since the cultivation of HuTu 80 cells started in 1968 and this cell line was directly deposited at ATCC by original developer in 1973, it is very provable that the AZ-521 is a subline derives from HuTu 80.
The AZ-521 has been widely used in the world and has been studied as standard cell strain for research of Helicobacter pilori. Therefore the cell bank does not exclude the AZ-521 from the catalogue. However, please consider and keep in mind in the usage of AZ-521 that the derivation of this cell line has problem.
(JCRB Cell Bank:http://cellbank.nibio.go.jp/~cellbank/cgi-bin/search_res_det.cgi?ID=237#)