細胞株の詳細データ

TKG 0185 ：： AZ521

ID：	TKG 0185
Cell name：	AZ521
Data accepted：
Animal：	Human.
Scientific name：	Homo sapiens.
Sex：
Case history：
Tissue：	Stomach.
Gentics：
Life span：	Infinite.
Morphology：	Epithelial-like.
Medium：	DMEM + 10% MEM + 10% FBS + NEAA.
Passage method：	0.02% EDTA-PBS or 0.25% trypsin.
Characteristics：	Gastric cancer. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature：
CO2 concentration：
Subculture frequency：
Classification：
Mycoplasma：
Isozyme analysis：
Established by：
References：	Br. J. Cancer, 59, 761-765, 1989. PMID: 2736210
References by users：
Comments：	AZ-521 cell line was reported to be established from human gastric carcinoma in Japan. However, the STR DNA analysis pattern of AZ-521 revealed to be identical to that of other cell line derived from human duodenum carcinoma, HuTu 80 in ATCC (registry number, HTB-40). This was confirmed by the Sanger Institute's analysis of identities of human cancer cell lines. http://www.sanger.ac.uk/genetics/CGP/CellLines/ Since the cultivation of HuTu 80 cells started in 1968 and this cell line was directly deposited at ATCC by original developer in 1973, it is very provable that the AZ-521 is a subline derives from HuTu 80. The AZ-521 has been widely used in the world and has been studied as standard cell strain for research of Helicobacter pilori. Therefore the cell bank does not exclude the AZ-521 from the catalogue. However, please consider and keep in mind in the usage of AZ-521 that the derivation of this cell line has problem. (JCRB Cell Bank:http://cellbank.nibio.go.jp/~cellbank/cgi-bin/search_res_det.cgi?ID=237#)
Deposited by：
Restriction：
DNA Profile （STR-PCR)	D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO 12, 13 8, 11 9, 11 10, 11 16, 18 7 XY 9, 11 11, 13
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