細胞株の詳細データ

TKG 0205 ：： HepG2

ID	TKG 0205
Cell name	HepG2
Data accepted	March, 1991.
Animal	Human. (male, 15 y. o.).
Scientific name	Homo sapiens.
Sex
Case history
Tissue	Liver.
Genetics
Life span	Infinite.
Morphology	Epithelial-like.
Medium	DMEM + 10% FBS (30% conditioned medium) or GIT.
Passage method	0.25% trypsin.
Characteristics	Hepatoma (hepato cellular), α-fetoprotein(+), albumin(+). HLA-A 02/2402. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature	37℃
CO2 concentration	5%
Subculture frequency	1:2 ~ 1:4, twice a week.
Classification
Mycoplasma	PCR: -, Hoechst DNA staining: -
Established by
References	Nature, 282, 615-616, 1979(PMID: 233137)., Science, 209, 497-499, 1980(PMID: 6248960)., In Vitro. Cell. Dev. Biol., 23, 349-354, 1987(PMID: 3034851).
References by users	Biochem. Biophys. Res. Commun., 249, 131-137, 1998(PMID: 9705844)., Biochem. Biopys. Res. Commun., , 270, 649-656, 2000(PMID: 10753678)., Int. J Oncology, 18, 1175-1179, 2001(PMID: 11351248)., Int. J. Mol. Med., 10, 287-291, 2002(PMID: 12165802)., Oncol. Rep., 9, 1233-1236, 2002(PMID: 12375026)., Hepatology, 37, 1086-1096, 2003(PMID: 12717389)., Oncol. Rep., 10, 415-420, 2003(PMID: 12579282)., Oncol. Rep., 10, 671-677, 2003(PMID: 12684642)., Int. Immunol., 16, 777-786, 2004(PMID: 15096476)., J. Biol. Chem., 282, 23532-23540, 2007(PMID: 17573348)., Biochem. Biophys. Res. Commun., 366, 110-116, 2008(PMID: 18053801)., J. Control. Release, 136, 213-219, 2009(PMID: 19233236), Cancer Med. 2013 Jun;2(3):305-15(PMID: 23930207), Toxicol Lett. 2015 Jan 22;232(2):393-402(PMID: 25448278), Chem Pharm Bull. 2018;66(6):682-687(PMID: 29863070), Technol Cancer Res Treat. 2020 Jan-Dec:19:1533033820980077(PMID: 33334271), J Radiat Res. 2020 Nov 16;61(6):819-827(PMID: 32880638).
Comments
Deposited by	Sagami, I.(RITC, Tohoku Univ.).
Restriction
DNA Profile (STR-PCR)	D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO 11,12 9,13 10 12,13 17 9 XY 8,9 10,11
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