細胞株の詳細データ

TKG 0205 :: HepG2

ID: TKG 0205
Cell name: HepG2
Data accepted: March, 1991.
Animal: Human. (male, 15 y. o.).
Scientific name: Homo sapiens.
Sex:
Case history:
Tissue: Liver.
Genetics:
Life span: Infinite.
Morphology: Epithelial-like.
Medium: DMEM + 10% FBS (30% conditioned medium) or GIT.
Passage method: 0.25% trypsin.
Characteristics: Hepatoma (hepato cellular), α-fetoprotein(+), albumin(+). HLA-A 02/2402. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature: 37℃
CO2 concentration: 5%
Subculture frequency:
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Established by:
References: Nature, 282, 615-616, 1979(PMID: 233137)., Science, 209, 497-499, 1980(PMID: 6248960)., In Vitro. Cell. Dev. Biol., 23, 349-354, 1987(PMID: 3034851).
References by users: Biochem. Biophys. Res. Commun., 249, 131-137, 1998(PMID: 9705844)., Biochem. Biopys. Res. Commun., , 270, 649-656, 2000(PMID: 10753678)., Int. J Oncology, 18, 1175-1179, 2001(PMID: 11351248)., Int. J. Mol. Med., 10, 287-291, 2002(PMID: 12165802)., Oncol. Rep., 9, 1233-1236, 2002(PMID: 12375026)., Hepatology, 37, 1086-1096, 2003(PMID: 12717389)., Oncol. Rep., 10, 415-420, 2003(PMID: 12579282)., Oncol. Rep., 10, 671-677, 2003(PMID: 12684642)., Int. Immunol., 16, 777-786, 2004(PMID: 15096476)., J. Biol. Chem., 282, 23532-23540, 2007(PMID: 17573348)., Biochem. Biophys. Res. Commun., 366, 110-116, 2008(PMID: 18053801)., J. Control. Release, 136, 213-219, 2009(PMID: 19233236).
Comments:
Deposited by: Sagami, I.(RITC, Tohoku Univ.).
Restriction:
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
11,12 9,13 10 12,13 17 9 XY 8,9 10,11
Photo: