ID: |
TKG 0206 |
Cell name: |
HuH-7 |
Data accepted: |
|
Animal: |
Human. |
Scientific name: |
Homo sapiens. |
Sex: |
|
Case history: |
|
Tissue: |
Liver.
|
Genetics: |
|
Life span: |
Infinite. |
Morphology: |
Epithelial-like.
|
Medium: |
RPMI-1640 + 10% FBS (30% conditioned med) or GIT.
|
Passage method: |
0.25% trypsin. |
Characteristics: |
Highly differentiated, hepatocelluler car., AFP+, ALB+, C3, C4.
|
Temperature: |
37℃ |
CO2 concentration: |
5% |
Subculture frequency: |
|
Classification: |
|
Mycoplasma: |
PCR: -, Hoechst DNA staining: - |
Established by: |
Nakabayashi, H.
|
References: |
Cancer Res., 42, 3858-3866, 1982., Cancer Res., 45, 6379-6383, 1985(PMID:
2415243)., Tiss. Cul. Res. Commun., 12, 221-227, 1993. |
References by users: |
J. Hepatolo., 27, 677-687, 1997(PMID: 9365044)., Hepatology, 33, 1087-1097, 2001(PMID: 11343236)., Mol. Cell Endocrinol., 231, 75-85, 2005(PMID: 15713537). |
Comments: |
|
Deposited by: |
Tanno, H. (1st Dept. Surg., Tohoku Univ.).
|
Restriction: |
|
DNA Profile
(STR-PCR) |
D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
12 |
10,11 |
11 |
10 |
16,18 |
7 |
X |
8,11 |
11 |
|
Photo: |
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