ID |
TKG 0217 |
Cell name |
L929 |
Data accepted |
|
Animal |
Mouse (C3H). |
Scientific name |
Mus musculus. |
Sex |
|
Case history |
|
Tissue |
Loose connective tissue.
|
Genetics |
|
Life span |
Infinite. |
Morphology |
Fibroblast-like. |
Medium |
RPMI-1640 + 10% FBS.
|
Passage method |
0.02% EDTA-PBS.
|
Characteristics |
Screening cell for TNF assay.
|
Temperature |
37℃ |
CO2 concentration |
5% |
Subculture frequency |
1:3 ~ 1:10, twice a week. |
Classification |
|
Mycoplasma |
PCR: -, Hoechst DNA staining: - |
Isozyme analysis |
LD, NP |
Established by |
Earle, W. R. (brought to Japan by Katsuta, H., 1955).
|
References |
J. Nat. Cancer Inst., 4, 165-212, 1943. J.
Nat. Cancer Inst., 12, 133-153, 1951PMID: 14874126 . Exp.
Cell Res., 26, 404-410, 1962.PMID: 14449548 Natl.
Cancer Inst. Monogr., 7, 147-158, 1962.PMID: 14006338 Soshiki-Baiyo-Kenkyu,
9 (No.2), 1-2, 1991.
|
References by users |
Int Immunopharmacol. 2007 Feb;7(2):191-7(PMID:17178386). |
Comments |
|
Deposited by |
Ohshima, H. (Teikyo Univ.) |
Restriction |
|
Photo |
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