細胞株の詳細データ
TKG 0233 :: NS-1(P3/NS1/1-Ag4-1)
ID | TKG 0233 |
Cell name | NS-1 (P3/NS1/1-Ag4-1) |
Data accepted | Nov., 1980. |
Animal | Mouse (BALB/c). |
Scientific name | Mus musculus. |
Sex | |
Case history | |
Tissue | B cell (myeloma), derived from MOPC-21. |
Genetics | |
Life span | Infinite. |
Morphology | Myeloma-like. |
Medium | RPMI-1640 + 10% FBS. |
Passage method | Dilute 1/8 every 3-4 days. |
Characteristics | Parent myeloma to construct mouse B cell hybridoma, HGPRT(-). Intracellular kappa chains are only secreted in a hybrid cell when attached to heavy chain. |
Temperature | 37℃ |
CO2 concentration | 5% |
Subculture frequency | |
Classification | |
Mycoplasma | PCR: -, Hoechst DNA staining: - |
Isozyme analysis | LD |
Established by | Kohler, G. & Milstein, C. |
References | Eur. J. Immunol., 6, 511, 1976.PMID: 825377 J. Mol. Biol., 60, 61, 1970. Exp. Cell. Res., 60, 61, 1970.PMID: 5439579 |
References by users | |
Comments | |
Deposited by | Uno, M. (Nihon Univ.). |
Restriction | |
Photo | Photo(X200) |