細胞株の詳細データ

TKG 0247 :: SKW-3

ID TKG 0247
Cell name SKW-3
Data accepted 1990.
Animal Human.
Scientific name Homo sapiens.
Sex
Case history
Tissue Blood.
Genetics
Life span Infinite.
Morphology Lymphocyte-like.
Medium RPMI-1640 + 10% FBS.
Passage method Dilute 1/5 every 3-4 days.
Characteristics Human T cell leukemia cell line (derivative of KE-37) originally described to be established from the peripheral blood of a 61-year-old man with T cell chronic lymphocytic leukemia (CLL) in 1977; KE-37 was established from a 27-year-old man with acute lymphoblastic leukemia (ALL) in 1979. CD4+, CD8+.
A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature 37℃
CO2 concentration 5%
Subculture frequency
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by
References Exp. Hematol., 9, 179-186, 1981(PMID: 6972317).
References by users Int. J. Hematology, 73, 507-516, 2001(PMID: 11503967).
Comments
Deposited by
Restriction
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
12, 13 8, 12 8, 12 11, 12 17, 18 6, 9.3 XY 8 10, 12
Photo
 Photo(x100)