細胞株の詳細データ

TKG 0277 :: TN-1

ID TKG 0277
Cell name TN-1
Data accepted
Animal Human.
Scientific name Homo sapiens.
Sex Male.
Age 2.y.o
Tissue Right adrenal gland.
Genetics
Life span Infinite.
Morphology Eipthelial-like.
Medium RPMI-1640 + 10% FBS. DM-160 + 15% FBS.
Passage method 0.25% trypsin.
Characteristics Neuroblastoma, transplantable to nude mouse, HVA production (+), DOPA production (+), noradrenaline production (+). Production of these amines is enhanced by dbc-AMP.  A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Culture DNA synthesis is enhanced by adding culture supernatant from bone marrow cell culture
Temperature 37℃
CO2 concentration 5%
Subculture frequency
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by Hayashi, T. (Pediat. Surg., Tohoku Univ.).
References Human Cell (Japanese), 2, 198, 1989(PMID: 2486611).
References by users J. Pediatric Hematlolgy Oncology, 22, 20-26, 2000(PMID: 10695817).
Comments
Deposited by
Restriction
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
10, 11 8, 13 11, 12 11, 12 14, 19 6 X 8, 11 11, 12
Photo

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