細胞株の詳細データ

TKG 0280 :: Vero

ID: TKG 0280
Cell name: Vero
Data accepted:
Animal: African green monkey.
Scientific name: Cercopithecus aethiops..
Sex:
Case history:
Tissue: Kidney.
Genetics:
Life span: Infinite.
Morphology: Fibroblast-like.
Medium: RPMI-1640 + 10% FBS (Med 199 + 5% FBS),or D-MEM+10%FBS.
Passage method: 0.25% trypsin.
Characteristics: This cell line has been employed extensively in virus replication and mycoplasma studies.  A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature: 37℃
CO2 concentration: 5%
Subculture frequency: 1:10 ~ 1:20, twice a week.
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Isozyme analysis: LD, NP
Established by: Yasumura, Y. & kawakita, Y. (Chiba U.).
References: Proc. Soc. Exp. Biol. Med., 125, 119, 1967. ibid. 127, 642, 1968. Nature, 216, 271, 1967.
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