細胞株の詳細データ

TKG 0282 :: WEHI164S

ID TKG 0282
Cell name WEHI164S
Data accepted Sep., 1991.
Animal Mouse (BALB/c).
Scientific name Mus musculus.
Sex
Case history
Tissue: Fibrosarcoma.
Genetics
Life span Finite.
Morphology Fibroblast-like.
Medium RPMI-1640 + 10% FBS.
Passage method 0.02% EDTA/PBS
Characteristics Useful for detection of TNF, very sensitive to TNF. Established by: Williamson, B.  A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:20, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Isozyme analysis LD
Established by
References J. Immunol., 141, 3422-3428, 1988.PMID: 2903192 J. Immunol. Meth., 95, 85-99, 1986.
References by users
Comments
Deposited by Ohta, N. (Med. Oncology, School of Med., Nagasaki Univ.)
Restriction In publishing the research results obtained by use this cell line, a citation of the following literature is necessary.
J. Immunol., 141, 3422-328, 1988.PMID: 2903192
Photo
 Photo(x100).