細胞株の詳細データ

TKG 0294 :: MG-63

ID TKG 0294
Cell name MG-63
Data accepted May, 1992.
Animal Human.
Scientific name Homo sapiens.
Sex
Case history
Tissue Osteosarcoma.
Genetics
Life span Infinite.
Morphology Epithelial-like.
Medium Eagle's MEM + 10% FBS or GIT.
Passage method 0.25% trypsin or 0.02% EDTA-PBS.
Characteristics 14 years old male, high yield of IFN production by induction with poly IC, cyclohexmide and actinomycin D. Antigenically, MG-63 IFN is closely related to human fibroblast IFN.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:10 ~ 1:20, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by
References Antimicrob. Agents. Chemother., 12, 11-15, 1977(PMID: 883813).
References by users Int. J. Mol. Med., 5, 505-510, 2000(PMID: 10762653), Toxicology Letters, 143, 1-7, 2003(PMID: 12697374), Bone. 2007 Apr;40(4):876-87(PMID: 17254854), Cancer Sci. 2008 Mar;99(3):518-23(PMID: 18081879), Lite Sci. 2016 Jun 15:155:29-35(PMID: 27177772), Int J Mol Sci. 2017 Oct 17;18(10):2159(PMID: 29039776).
Comments
Deposited by Asakura, S. (Jichi Med. College).
Restriction
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
11, 12 11 10 11 16, 19 9.3 XY 8, 11 10, 12
Photo