ID |
TKG 0302 |
Cell name |
HMV-I |
Data accepted |
Feb., 3, 1986. |
Animal |
Human. |
Scientific name |
Homo sapiens. |
Sex |
|
Case history |
|
Tissue |
Melanoma (vagina origin).
|
Gentics |
|
Life span |
Infinite. |
Morphology |
Epithelial-like.
|
Medium |
DMEM/F12 + 10% FBS. |
Passage method |
0.25% trypsin. |
Characteristics |
Human melanoma, melanin production (-). Evidence from DNA fingerprinting
indicates that this line and HeLa are derived from the same individual. A
culture submitted to the Cell Resource Center for Biomedical Research was
found to be contaminated with mycoplasma. Progeny were cured by Treatments
with BM Cyclin and MC210. |
Temperature |
|
CO2 concentration |
|
Subculture frequency |
|
Classification |
|
Mycoplasma |
PCR: - |
Established by |
Kasuga, T.
|
References |
Cell
Differentiation, 1, 149-155, 1972.PMID: 4209534 Biology of Normal and
Abnormal Melanocytes. (Kawamura ed., university Tokyo Press), pp. 241-264, 1971.
Jingansaibou no baiyo, pp. 388, 1975 (Asakura Press). Cancer,
42, 2675-2686, 1978.PMID: 365316
|
References by users |
|
Comments |
|
Deposited by |
Oikawa, A. (Inst. Develop. Aging and Cancer, Tohoku Univ.). |
Restriction |
|
DNA Profile
(STR-PCR) |
D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
11, 12 |
13.3 |
8, 12 |
9 |
16, 18, 19 |
7 |
X |
8, 12 |
9, 10 |
|
Photo |
|