細胞株の詳細データ

TKG 0304 :: KU812-F

ID: TKG 0304
Cell name: KU812-F
Data accepted: Nov., 1991.
Animal: Human (38 y. o. male).
Scientific name: Homo sapiens.
Sex:
Case history:
Tissue: Blood (leukemia cells).
Genetics:
Life span: Infinite.
Morphology: Lymphoblast-like (myeloblast-like), adherent.
Medium: RPMI-1640 + 10% FBS.
Passage method: Dilute 1/5 every 3-4 days.
Characteristics: Human basophilic leukemia. In serum-free medium, this cell line differentiates to basophilic cells.  A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature: 37℃
CO2 concentration: 5%
Subculture frequency:
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Established by: Kishi, K. (Niigata U.).
References: Leuk. Res., 9, 381-390, 1985.PMID: 3858609 Leuk. Res., 11, 1105-1113, 1987.PMID: 3695564 Blood, 69, 1414-1418, 1987.PMID: 2436686 Blood, 70, 612, 1987.PMID: 3304457
References by users:
Comments:
Deposited by: Fukuda, T. (Nigata U., Dep. Pathol.), Tanno, Y., Yamauchi, K. (1st. Dep. Internal Med., Tohoku U.).
Restriction:
DNA Profil
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
10 8 12 9 15 9 XY 12 12, 13
Photo: