細胞株の詳細データ

TKG 0306 :: 293(CRL1573)

ID: TKG 0306
Cell name: 293 (CRL1573)
Data accepted:
Animal: Human.
Scientific name: Homo sapiens.
Sex:
Case history:
Tissue: Primary embryonal kidney (human).
Gentics:
Life span: Infinite.
Morphology:
Medium: Eagle's MEM + 10% FBS.
Passage method: 0.25% trypsin.
Characteristics: Primary human kidney (embryonal) transformed by sheared human adenovirus type 5 (Ad 5) DNA, contain and express the transfrming genes of Ad 5. Handle as potentially biohazadeous material under Biosafety level 2. Excellent for titrating human adenoviruses.  A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature:
CO2 concentration:
Subculture frequency:
Classification:
Mycoplasma: PCR: -
Established by:
References: J. Gen. Virol., 36, 59-74, 1977(PMID: 886304)., Virology, 77, 319-329, 1977(PMID: 841862)., Virology, 86, 10-21, 1978(PMID: 664220).
References by users: Br. J. Radiology, 75, 657-662, 2002(PMID: 12153939)., Nat. Methods, 7, 729-732, 2010(PMID: 20693999).
Comments:
Deposited by:
Restriction:
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
8, 9 12 11, 12 9, 13 16, 19 7, 9.3 X 11 11, 12
Photo: