ID |
TKG 0308 |
Cell name |
BHK-21 |
Data accepted |
|
Animal |
Hamster (Syrian or golden) |
Scientific name |
Mesocrcetus auratus |
Sex |
|
Case history |
|
Tissue |
Kidneys of five unsexed, 1-day-old hamsters |
Genetics |
|
Life span |
Infinite |
Morphology |
Fibroblast-like, pseudodiploi.
|
Medium |
MEM + 10% newborn bovine serum (or FBS). |
Passage method |
|
Characteristics |
This cell line has been used for polyoma virus transformation, foot-and
mouth disease, rabies, and many arbovirus replication studies. A culture
submitted to the Cell Resource Center for Biomedical Research was found
to be contaminated with mycoplasma. Progeny were cured by Treatments with
BM Cyclin and MC210. |
Temperature |
37℃ |
CO2 concentration |
5% |
Subculture frequency |
1:9, twice a week. |
Classification |
|
Mycoplasma |
PCR: -, Hoechst DNA staining: - |
Isozyme analysis |
LD |
Established by |
Macpherson, I. A. & Stocker, M. G. P. (1961).
|
References |
Virologoy, 16, 147, 1962. J. Natl. Cancer Inst., 30, 795, 1963.
|
References by users |
|
Comments |
|
Deposited by |
Kan, M. (RITC, Tohoku U.).
|
Restriction |
|
Photo |
(x100) |