細胞株の詳細データ

TKG 0308 :: BHK-21

ID TKG 0308
Cell name BHK-21
Data accepted
Animal Hamster (Syrian or golden)
Scientific name Mesocrcetus auratus
Sex
Case history
Tissue Kidneys of five unsexed, 1-day-old hamsters
Genetics
Life span Infinite
Morphology Fibroblast-like, pseudodiploi.
Medium MEM + 10% newborn bovine serum (or FBS).
Passage method
Characteristics This cell line has been used for polyoma virus transformation, foot-and mouth disease, rabies, and many arbovirus replication studies. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:9, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Isozyme analysis LD
Established by Macpherson, I. A. & Stocker, M. G. P. (1961).
References Virologoy, 16, 147, 1962. J. Natl. Cancer Inst., 30, 795, 1963.
References by users
Comments
Deposited by Kan, M. (RITC, Tohoku U.).
Restriction
Photo (x100)