細胞株の詳細データ

TKG 0318 :: HMV-II

ID: TKG 0318
Cell name: HMV-II
Data accepted: Aug., 7, 1987.
Animal: Human.
Scientific name: Homo sapiens.
Sex:
Case history:
Tissue: Vagina melanoma (65 y. o.).
Genetics:
Life span: Infinite.
Morphology: Spindle-like.
Medium: F12 (or MEM/F12) + 10% FBS.
Passage method: 0.25% trypsin.
Characteristics: Melanotic melanoma (highly melanotic).  A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature: 37℃
CO2 concentration: 5%
Subculture frequency: 1:1 ~ 1:2, twice a week.
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining; -
Established by: Kasuga, T. (Prof. Dermatol., Tokyo Med. Dent. Collge).
References: Cell Differentiation, 1, 149-155, 1972(PMID: 4209534)., Jingansaibou no baiyo (Ohboshi and Sugano ed. Asakura Publisher, 1975) pp. 203-208. Cancer, 42, 2675-2686, 1978(PMID: 365316).,
References by users: Biochim. Biophys. Acta., 1009, 283-286, 1989(PMID: 2480811)., Chem. Pharm. Bull, 51, 798-801, 2003(PMID: 12843585)., Anticancer Res., 26, 4349-4356, 2006(PMID: 17201154).
Comments:
Deposited by: Saeki, H. (CCR. Tohoku Univ.)
Restriction:
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
12 11 8, 14 11 16, 17 6, 9 X 8 13
Photo: