細胞株の詳細データ

TKG 0328 :: CHO-K1

ID: TKG 0328
Cell name: CHO-K1
Data accepted: Nov., 1987.
Animal: Chinese hamster.
Scientific name: Cricetulus griseus.
Sex:
Case history:
Tissue: Ovary.
Genetics:
Life span: Infinite.
Morphology: Epithelial-like.
Medium: F12 medium + 10% FBS.
Passage method: 0.25% trypsin.
Characteristics: This cell line was derived as subclone from parental CHO cell line initiated from a biopsy of an ovary of an adult hamster. This cell line has a requirement for proline. Useful for induction and isolation of nutritionally deficient mutants and for regulation of gene expression through hybridization.  A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature: 37℃
CO2 concentration: 5%
Subculture frequency:
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Isozyme analysis: LD
Established by: Puck, T. T.
References: J. Exp. Med., 108, 945, 1958(PMID: 13598821)., Proc. NAS., 52, 288, 1965., Genetics, 55, 513, 1967(PMID: 6068403)., Proc. NAS., 60, 1275, 1968. Science, 164, 312, 1969(PMID: 5813351).
References by users: Mol. Cell Biol., 25, 4388-4396, 2005(PMID: 15899845)., J. Pept. Sci., 14, 261-266, 2008(PMID 17880034)
Comments:
Deposited by: Pharmacol. (Inst. Develop. Aging and Cancer, Tohoku Univ.).
Restriction:
Photo:       
 Photo(x100)                       Photo(x200)