ID |
TKG 0346 |
Cell name |
B16C2M |
Data accepted |
|
Animal |
Mouse (C57BL/6J).
|
Scientific name |
Mus musculus. |
Sex |
|
Case history |
|
Tissue |
Melanoma. |
Genetics |
|
Life span |
Infinite. |
Morphology |
Epithelial-like.
|
Medium |
RPMI-1640 + 10% FBS. |
Passage method |
0.25% trypsin.
|
Characteristics |
Melanotic, cloned twice. A culture submitted to the Cell Resource Center
for Biomedical Research was found to be contaminated with mycoplasma. Progeny
were cured by Treatments with BM Cyclin and MC210. |
Temperature |
37℃ |
CO2 concentration |
5% |
Subculture frequency |
1:20, twice a week. |
Classification |
|
Mycoplasma |
PCR: -, Hoechst DNA staining: - |
Isozyme analysis |
LD |
Established by |
Oikawa, A. |
References |
Cell
differentiation, 1, 149-155, 1972.PMID: 4209534
|
References by users |
|
Comments |
|
Deposited by |
Oikawa, A. (RITC).
|
Restriction |
|
Photo |
|