細胞株の詳細データ

TKG 0346 :: B16C2M

ID TKG 0346
Cell name B16C2M
Data accepted
Animal Mouse (C57BL/6J).
Scientific name Mus musculus.
Sex
Case history
Tissue Melanoma.
Genetics
Life span Infinite.
Morphology Epithelial-like.
Medium RPMI-1640 + 10% FBS.
Passage method 0.25% trypsin.
Characteristics Melanotic, cloned twice.  A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:20, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Isozyme analysis LD
Established by Oikawa, A.
References Cell differentiation, 1, 149-155, 1972.PMID: 4209534
References by users
Comments
Deposited by Oikawa, A. (RITC).
Restriction
Photo