| ID： |
TKG 0347 |
| Cell name： |
B16F1 |
| Data accepted： |
June, 1988. |
| Animal： |
Mouse (C57BL/6J). |
| Scientific name： |
Mus musculus. |
| Sex： |
|
| Case history： |
|
| Tissue： |
Melanoma. |
| Genetics： |
|
| Life span： |
Infinite. |
| Morphology： |
Epithelial-like.
|
| Medium： |
RPMI-1640 + 10% FBS. |
| Passage method： |
0.25% trypsin. |
| Characteristics： |
Useful for metastasis study. A culture submitted to the Cell Resource
Center for Biomedical Research was found to be contaminated with mycoplasma.
Progeny were cured by Treatments with BM Cyclin and MC210. |
| Temperature： |
37℃ |
| CO2 concentration： |
5% |
| Subculture frequency： |
1:20, twice a week. |
| Classification： |
|
| Mycoplasma： |
PCR: -, Hoechst DNA staining: - |
| Isozyme analysis： |
LD |
| Established by： |
Fidler, I. J.
|
| References： |
Nature
New Biology, 242, 148-149, 1973.PMID: 4512654
|
| References by users： |
|
| Comments： |
|
| Deposited by： |
Dep. Radiology, RITC. |
| Restriction： |
|
| Photo： |
 |
