ID: |
TKG 0347 |
Cell name: |
B16F1 |
Data accepted: |
June, 1988. |
Animal: |
Mouse (C57BL/6J). |
Scientific name: |
Mus musculus. |
Sex: |
|
Case history: |
|
Tissue: |
Melanoma. |
Genetics: |
|
Life span: |
Infinite. |
Morphology: |
Epithelial-like.
|
Medium: |
RPMI-1640 + 10% FBS. |
Passage method: |
0.25% trypsin. |
Characteristics: |
Useful for metastasis study. A culture submitted to the Cell Resource
Center for Biomedical Research was found to be contaminated with mycoplasma.
Progeny were cured by Treatments with BM Cyclin and MC210. |
Temperature: |
37℃ |
CO2 concentration: |
5% |
Subculture frequency: |
1:20, twice a week. |
Classification: |
|
Mycoplasma: |
PCR: -, Hoechst DNA staining: - |
Isozyme analysis: |
LD |
Established by: |
Fidler, I. J.
|
References: |
Nature
New Biology, 242, 148-149, 1973.PMID: 4512654
|
References by users: |
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Comments: |
|
Deposited by: |
Dep. Radiology, RITC. |
Restriction: |
|
Photo: |
 |