細胞株の詳細データ
TKG 0354 :: TKC2
ID | TKG 0354 |
Cell name | TKC2. |
Data accepted | Feb. 1992. |
Animal | C57BL/6 mouse. |
Scientific name | Mus musculus. |
Sex | |
Case history | |
Tissue | Kindney. |
Genetics | |
Life span | Infinite. |
Morphology | Epitherial-like, multicellular domes are observed at a confluence. |
Medium | RITC80-7 + 5% FBS +Tf (10μg/ml) + Ins (1μg/ml) + EGF (10ng/ml). |
Passage method | 0.25% trypsin |
Characteristics | Established from tsSV40 large T-antigen gene transfected mouse kidney. Culture at 33℃, using collagen-coaated dish. The level of cAMP accumulation stimulated by vasopressin in this cell line is equivalent to those of MDCK cells which were originated from a distal tubule cells of nephron segments. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210. |
Temperature | 33℃ |
Survival | |
CO2 concentration | 5% |
Subculture frequency | 1:5 ~ 1:10, twice a week. |
Classification | |
Mycoplasma | PCR: -, Hoechst DNA staining: ± |
Isozyme analysis | LD, NP |
Established by | Satoh, T. The cell were propagated and maintained in the cell bank Tohoku. |
References | Jpn. J. Cancer Res., 82, 1344-1348, 1991.PMID: 1663919 |
References by users | |
Comments | |
Deposited by | Yanai, N. (RITC) |
Restriction | In publishing the research results obtained by use this cell line, a citation of the following literature is necessary. Jpn. J. Cancer Res., 82, 1344-1348, 1991.PMID: 1663919 |
Photo | Photo(x100) |