細胞株の詳細データ

TKG 0354 :: TKC2

ID TKG 0354
Cell name TKC2.
Data accepted Feb. 1992.
Animal C57BL/6 mouse.
Scientific name Mus musculus.
Sex
Case history
Tissue Kindney.
Genetics
Life span Infinite.
Morphology Epitherial-like, multicellular domes are observed at a confluence.
Medium RITC80-7 + 5% FBS +Tf (10μg/ml) + Ins (1μg/ml) + EGF (10ng/ml).
Passage method 0.25% trypsin
Characteristics Established from tsSV40 large T-antigen gene transfected mouse kidney. Culture at 33℃, using collagen-coaated dish. The level of cAMP accumulation stimulated by vasopressin in this cell line is equivalent to those of MDCK cells which were originated from a distal tubule cells of nephron segments. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature 33℃
Survival
CO2 concentration 5%
Subculture frequency 1:5 ~ 1:10, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: ±
Isozyme analysis LD, NP
Established by Satoh, T. The cell were propagated and maintained in the cell bank Tohoku.
References Jpn. J. Cancer Res., 82, 1344-1348, 1991.PMID: 1663919
References by users
Comments
Deposited by Yanai, N. (RITC)
Restriction In publishing the research results obtained by use this cell line, a citation of the following literature is necessary.
Jpn. J. Cancer Res., 82, 1344-1348, 1991.PMID: 1663919
Photo
 Photo(x100)