細胞株の詳細データ

TKG 0379 :: DLD-1

ID TKG 0379
Cell name DLD-1
Data accepted Aug., 1993
Animal Human.
Scientific name Homo sapiens
Sex
Case history
Tissue Colon
Genetics
Life span Infinite
Morphology Epithelial-like
Medium RPMI-1640 + 10% FBS
Passage method 0.05% trypsin.
Characteristics Colorectal adenocarcinoma cell line. This cell line is a well-characterized cell line in studies of the effects of polar solvents. APC: One nucleotide deletion at 4251. Truncated APC protein detected in the cytoplasmic skeleton that is not recognized by antibodies against C-terminal portion. p53: C→T; Ser241→Phe. Evidence from DNA fingerprinting indicates that this line and HCT-15 are derived from the same individual.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:10 ~ 1:20, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by D. L. Dexter.
References Cancer Res., 39, 1020-1025, 1979(PMID: 427742)., Proc. Natl. Acad. Sci. USA., 87, 7555-7559, 1990(PMID: 1699228)., Proc. Natl. Acad. Sci. USA., 94, 3034-3039, 1997(PMID: 9096341).
References by users Proc. Natl. Acad. Sci., 96, 4874-4973, 1999(PMID: 10220386)., Biochem. Biopys. Res. Commun., , 270, 649-656, 2000(PMID: 10753678)., Int. Immunol., 16, 777-786, 2004(PMID: 15096476)., Pharm Res. 2010 Mar;27(3):510-6(PMID: 20130966), Oncol Rep. 26: 949-955, 2011 (PMID: 21743971), Biochim Biophys Acta 1821 (2012) 980-988 (PMID: 22521763), Pharm Res. 2013 Nov;30(11):2880-90(PMID: 23812637), Cancer Med. 2013 Jun;2(3):305-15(PMID: 23930207), J Nutr Biochem 26 (2015) 832-840 (PMID: 25979648), Mol Med Rep 19: 2767-2773, 2019 (PMID: 30720142), Int J Mol Med. 2023 Feb;51(2):18.(PMID: 36660940).
Comments
Deposited by Numasaki, M. (IDAC).
Restriction
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
13 8, 11 10, 12 12, 13 18, 19 7, 9.3 XY 8, 11 12
Photo