| ID |
TKG 0379 |
| Cell name |
DLD-1 |
| Data accepted |
Aug., 1993 |
| Animal |
Human. |
| Scientific name |
Homo sapiens |
| Sex |
|
| Case history |
|
| Tissue |
Colon |
| Genetics |
|
| Life span |
Infinite |
| Morphology |
Epithelial-like |
| Medium |
RPMI-1640 + 10% FBS |
| Passage method |
0.05% trypsin. |
| Characteristics |
Colorectal adenocarcinoma cell line. This cell line is a well-characterized cell
line in studies of the effects of polar solvents. APC: One nucleotide deletion
at 4251. Truncated APC protein detected in the cytoplasmic skeleton that is not
recognized by antibodies against C-terminal portion. p53: C→T; Ser241→Phe.
Evidence from DNA fingerprinting indicates that this line and HCT-15 are derived
from the same individual. |
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:10 ~ 1:20, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Established by |
D. L. Dexter.
|
| References |
Cancer Res., 39, 1020-1025, 1979(PMID: 427742)., Proc. Natl. Acad. Sci. USA., 87, 7555-7559, 1990(PMID: 1699228)., Proc. Natl. Acad. Sci. USA., 94, 3034-3039, 1997(PMID: 9096341). |
| References by users |
Proc. Natl. Acad. Sci., 96, 4874-4973, 1999(PMID: 10220386)., Biochem. Biopys. Res. Commun., , 270, 649-656, 2000(PMID: 10753678)., Int. Immunol., 16, 777-786, 2004(PMID: 15096476). |
| Comments |
|
| Deposited by |
Numasaki, M. (IDAC).
|
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 13 |
8, 11 |
10, 12 |
12, 13 |
18, 19 |
7, 9.3 |
XY |
8, 11 |
12 |
|
| Photo |
 |
