| ID |
TKG 0401 |
| Cell name |
KB |
| Data accepted |
July, 1994. |
| Animal |
Human. |
| Scientific name |
Homo sapiens. |
| Sex |
|
| Case history |
|
| Tissue |
Oral, Epidermoid carcinoma. |
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Epithelial-like |
| Medium |
MEM + 10% FBS. |
| Passage method |
0.02% EDTA-PBS. |
| Characteristics |
This cell line was derived from an epidermoid carcinoma in the mouth of
adult male Caucasian in 1954. It was one of the early successful attempts
to isolate and serially propagate a human cell line directly in monolayer
culture on glass. This line has been used extensively in studies of cell
nutrition and metabolism, cancer chemotherapy screening, tumorigenicity,
and viruses. This cell line has HeLa markers. A culture submitted to the
Cell Resource Center for Biomedical Research was found to be contaminated
with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210. |
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:5 ~ 1:10, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Established by |
H. Eagle. |
| References |
Proc. Soc. Exp. Biol. Med., 89, 362, 1955(PMID: 13254761)., Proc. Soc. Exp. Biol. Med., 91, 361-364, 1956(PMID: 13322936)., Proc. Soc. Exp. Biol. Med., 94, 661-664, 1957(PMID: 13431913)., Cancer Res., 18, 1017, 1958(PMID: 13596943)., Science, 133, 1559, 1961(PMID: 13745868). |
| References by users |
Int. J. Mol. Med., 10, 287-291, 2002(PMID: 12165802)., Oncol. Rep., 9, 1233-1236, 2002(PMID: 12375026)., Oncol. Rep., 10, 415-420, 2003(PMID: 12579282)., Oncol. Rep., 10, 671-677, 2003(PMID: 12684642). |
| Comments |
|
| Deposited by |
Suzuki, M. (Otolaryngol. Tohoku Univ.). |
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 11, 12 |
12, 13.3 |
8, 12 |
9, 10 |
16, 18 |
7 |
X |
8, 12 |
9, 10 |
|
| Photo |
 (x100) |
