細胞株の詳細データ

TKG 0401 :: KB

ID TKG 0401
Cell name KB
Data accepted July, 1994.
Animal Human.
Scientific name Homo sapiens.
Sex
Case history
Tissue Oral, Epidermoid carcinoma.
Genetics
Life span Infinite.
Morphology Epithelial-like
Medium MEM + 10% FBS.
Passage method 0.02% EDTA-PBS.
Characteristics This cell line was derived from an epidermoid carcinoma in the mouth of adult male Caucasian in 1954. It was one of the early successful attempts to isolate and serially propagate a human cell line directly in monolayer culture on glass. This line has been used extensively in studies of cell nutrition and metabolism, cancer chemotherapy screening, tumorigenicity, and viruses. This cell line has HeLa markers.  A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:5 ~ 1:10, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by H. Eagle.
References Proc. Soc. Exp. Biol. Med., 89, 362, 1955(PMID: 13254761)., Proc. Soc. Exp. Biol. Med., 91, 361-364, 1956(PMID: 13322936)., Proc. Soc. Exp. Biol. Med., 94, 661-664, 1957(PMID: 13431913)., Cancer Res., 18, 1017, 1958(PMID: 13596943)., Science, 133, 1559, 1961(PMID: 13745868).
References by users Int. J. Mol. Med., 10, 287-291, 2002(PMID: 12165802)., Oncol. Rep., 9, 1233-1236, 2002(PMID: 12375026)., Oncol. Rep., 10, 415-420, 2003(PMID: 12579282)., Oncol. Rep., 10, 671-677, 2003(PMID: 12684642).
Comments
Deposited by Suzuki, M. (Otolaryngol. Tohoku Univ.).
Restriction
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
11, 12 12, 13.3 8, 12 9, 10 16, 18 7 X 8, 12 9, 10
Photo (x100)