細胞株の詳細データ

TKG 0403 :: HEp-2

ID: TKG 0403
Cell name: HEp-2
Data accepted: July, 1994.
Animal: Human.
Scientific name: Homo sapiens.
Sex:
Case history:
Tissue: Larynx, Epidermoid carcinoma
Genetics:
Life span: Infinite.
Morphology: Epithelial-like
Medium: MEM + 10% FBS.
Passage method: 0.02% EDTA-PBS
Characteristics: This cell line was established from tumors that had been produced in irradiated-cortisinized weanling rats after injection with epidermoid carcinoma tissue from the larynx of a 56-y-o Caucasian male. The in vitro isolation was accomplished in each of several mixtures of bovine amniotic fluid, embryo extracts, human and horse sera, and balanced salt solution, and the epithelial-like cells subsequently grew well in several types of culture media. It is a hardy cell line which resists temperature, nutritional, and environmental changes without loss of viability. This line supports growth of arvoviruses, and measles virus. This line has HeLa markers.
Temperature: 37℃
Survival
CO2 concentration: 5%
Subculture frequency:
Classification:
Mycoplasma: PCR: -
Established by:
References: Cancer Res., 14, 660, 1954(PMID: 13209540)., Cancer Res., 15, 598, 1955(PMID: 13261081)., Proc. Soc. Exp. Biol. Med., 93, 107, 1956(PMID: 13370591)., Texas Rep. Biol. Med., 15, 588, 1957(PMID: 13495958)., Ann. N.Y. Acad. Sci., 76, 497, 1958(PMID: 13627875).
References by users: Gene Therapy, 10, 51-58, 2003(PMID: 12525837).
Comments:
Deposited by: Suzuki, M. (Otolaryngol. Tohoku Univ.).
Restriction:
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
11, 12 12, 13.3 8, 12 9, 10 16, 18 7 X 8, 12 9, 10
Photo: