ID |
TKG 0403 |
Cell name |
HEp-2 |
Data accepted |
July, 1994. |
Animal |
Human. |
Scientific name |
Homo sapiens.
|
Sex |
|
Case history |
|
Tissue |
Larynx, Epidermoid carcinoma |
Genetics |
|
Life span |
Infinite.
|
Morphology |
Epithelial-like |
Medium |
MEM + 10% FBS. |
Passage method |
0.05% trypsin. |
Characteristics |
This cell line was established from tumors that had been produced in
irradiated-cortisinized weanling rats after injection with epidermoid carcinoma
tissue from the larynx of a 56-y-o Caucasian male. The in vitro isolation was
accomplished in each of several mixtures of bovine amniotic fluid, embryo
extracts, human and horse sera, and balanced salt solution, and the
epithelial-like cells subsequently grew well in several types of culture media.
It is a hardy cell line which resists temperature, nutritional, and
environmental changes without loss of viability. This line supports growth of
arvoviruses, and measles virus. This line has HeLa markers. |
Temperature |
37℃ |
Survival |
|
CO2 concentration |
5% |
Subculture frequency |
1:4 ~ 1:9, twice a week. |
Classification |
|
Mycoplasma |
PCR: -, Hoechst DNA staining: - |
Established by |
|
References |
Cancer Res., 14, 660, 1954(PMID: 13209540)., Cancer Res., 15, 598, 1955(PMID: 13261081)., Proc. Soc. Exp. Biol. Med., 93, 107, 1956(PMID: 13370591)., Texas Rep. Biol. Med., 15, 588, 1957(PMID: 13495958)., Ann. N.Y. Acad. Sci., 76, 497, 1958(PMID: 13627875). |
References by users |
Gene Therapy, 10, 51-58, 2003(PMID: 12525837). |
Comments |
|
Deposited by |
Suzuki, M. (Otolaryngol. Tohoku Univ.).
|
Restriction |
|
DNA Profile
(STR-PCR) |
D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
11, 12 |
12, 13.3 |
8, 12 |
9, 10 |
16, 18 |
7 |
X |
8, 12 |
9, 10 |
|
Photo |
|