ID |
TKG 0411 |
Cell name |
H-111-TC |
Data accepted |
Dec. 1994. |
Animal |
Human. |
Scientific name |
Homo sapiens. |
Sex |
|
Case history |
|
Tissue |
gastric cancer. |
Genetics |
|
Life span |
Infinite. |
Morphology |
Epithelial-like, island-like |
Medium |
RPMI-1640 +10% FBS, Pc and SM. |
Passage method |
0.25% trypsin-EDTA-PBS. |
Characteristics |
Tissue culture cell line derived from nude mouse xenograft tumor (TKG 0342,
H-111). slow growing. We suggest to use type I collagen plate (6 well, Sumitomo
bakelite, celltight C-1) for better cultre. |
Temperature |
37℃ |
CO2 concentration |
5% |
Subculture frequency |
1:1, once or twice a week. |
Classification |
|
Mycoplasma |
PCR: -, Hoechst DNA staining: - |
Isozyme analysis |
LD |
Established by |
Kudo, T. (CCR, IDAC). |
References |
|
References by users |
Proc. Natl. Acad. Sci., 107, 17309-17314, 2010(PMID: 20855608). |
Comments |
|
Deposited by |
|
Restriction |
|
DNA Profile
(STR-PCR) |
D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
12 |
8 |
8, 11 |
10 |
17, 18 |
6 |
X |
8, 11 |
9 |
|
Photo |
|