細胞株の詳細データ

TKG 0457 :: CoLo 205

ID TKG 0457
Cell name CoLo 205
Data accepted April 1996.
Animal Human.
Scientific name Homo sapiens.
Sex
Case history
Tissue Colon, adenocarcinoma.
Genetics
Life span Infinite.
Morphology Epithelial-like with suspension cells.
Medium RPMI-1640 + 10% FBS.
Passage method 0.02% EDTA-PBS.
Characteristics Established from ascitic fluid of a 70-y-o male with carcinoma of colon. CEA-producing. transplantable to SCID mouse, subcutanousl and intraperitoneally. HLA-A 0101/0201
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:4~1:10, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by T.U. Semple.
References Cancer Res., 38, 1345-1355, 1978(PMID: 565251).
References by users Proc. Natl. Acad. Sci., 96, 4874-4973, 1999(PMID: 10220386)., Proc. Natl. Acad. Sci., 99, 10718-10723, 2002(PMID: 12149448)., Biochem. J., 394, 647-656, 2006(PMID: 16241905)., Cancer Sci, 101, 1822-1827, 2010(PMID: 20500518), Cancer Med. 2013 Jun;2(3):305-15(PMID: 23930207), Curr Issues Mol Biol. 2023 Apr 20;45(4):3658-3673.(PMID: 37185762), Int J Mol Med. 2023 Feb;51(2):18.(PMID: 36660940), Int J Mol Sci. 2023 May 7;24(9):8411(PMID: 37176118).
Comments
Deposited by
Restriction
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
10, 13 10, 12 9, 10 12, 13 15 8, 9 X 11 11, 12
Photo (x100)