ID |
TKG 0465 |
Cell name |
HeLa.P3(S) |
Data accepted |
|
Animal |
Human |
Scientific name |
Homo sapiens |
Sex |
|
Case history |
|
Tissue |
Cervix |
Genetics |
|
Life span |
Infinite |
Morphology |
Epithelial-like, partly floating like lymphoblast cells
|
Medium |
DM160 or RPMI 1640 + 10% FBS
|
Passage method |
0.02% EDTA-PBS
|
Characteristics |
Cervical cancer, culturable with serum-free medium. Established from 31-y-o-,
female. |
Temperature |
37℃ |
CO2 concentration |
5% |
Subculture frequency |
1:20 ~ 1:40, twice a week. |
Classification |
|
Mycoplasma |
PCR: -, Hoechst DNA staining: - |
Established by |
Katsuta H.
|
References |
Soshikibaiyou,
9, 196-200, 1983.PMID: 6350668 Jpn.
J. Exp. Med., 29, 493-505, 1959.PMID: 14404771 |
References by users |
|
Comments |
|
Deposited by |
|
Restriction |
|
DNA Profile
(STR-PCR) |
D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
11, 12 |
13.3 |
8, 12 |
9, 10 |
16, 18, 19 |
7 |
X |
8, 12 |
9, 10 |
|
Photo |
|