細胞株の詳細データ

TKG 0478 :: L1210 TGR4

ID: TKG 0478
Cell name: L1210 TGR4
Data accepted: April, 1996.
Animal: DBA/2 mouse.
Scientific name: Mus musculus.
Sex:
Case history:
Tissue: Leukemia.
Genetics:
Life span: Infinite.
Morphology: Lymphoblastic.
Medium: RPMI-1640 + 10% FBS, 10 μg 6TG/ml).
Passage method: Dilute 1/4 every 3-4 days.
Characteristics: This line was selected by the treatment of EMS from L1210. Resistant to 6TG (10 microgram/ml). This line is more immunogenic than the parent line and incapable of forming ascites tumors in BDF1 mice. 86 KDa (p86) protein, specific to this line, was identified as tumor rejection antigen by the studies of cell fusion between L1210 TGR4 and parent line. Immune-response directed to the specific p86 on L1210 TGR4 may be involved in the loss of tumorigenicity of this line.
Survival
CO2 concentration: 5%
Subculture frequency:
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Isozyme analysis: LD
Established by: Noriko Yamada (1st. Biochem. Kanazawa Medical Univ. Uchinada, Ishikawa).
References: 4st Int. Congress of Cell Biol. Aug. 1988. Abstract, p46.
References by users:
Comments:
Deposited by: Noriko Yamada
Restriction:
Photo: