細胞株の詳細データ

TKG 0479 :: MCF7

ID: TKG 0479
Cell name: MCF7
Data accepted:
Animal: Human.
Scientific name: Homo sapiens.
Sex:
Case history:
Tissue: Breast adenocarcinoma.
Genetics:
Life span: Infinite.
Morphology: Epithelial-like
Medium: E-MEM supplemented with non-essential AA, sodium pyruvate and 10% FBS, or RPMI-1640 + 10% FBS.
Passage method: 0.02% EDTA-PBS.
Characteristics: Established from pleural effusion of breast adenocarcinoma. Patint data: 69-y.o., female, Caucasian, O-type blood. This cell line retains several characteristics of differentiated mammary epithelium including ability to process estradiol via cytoplasmic estrogen receptors and forming domes.  A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
HLA profile: A2, A34, B12, B15 (HLA-A2/-, B18/44 and Cw0501/-).
CO2 concentration: 5%
Subculture frequency:
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Established by: H.D. Soule
References: J. Natl. Cancer Inst(Bethesda), 51, 1409-1416, 1973(PMID: 4357757)., Int. J. Cancer, 68, 535-546, 1996(PMID: 8945627).
References by users: Int. J. Cancer, 80, 64-67, 1999(PMID: 9935232)., Biochem. Biopys. Res. Commun., , 270, 649-656, 2000(PMID: 10753678)., J. Biol. Chem, 276, 42908-42914, 2001(PMID: 11555647)., Blood, 99, 4070-4078, 2002(PMID: 12010809)., Endocrinology, 143, 2635-2642, 2002(PMID: 12072396)., Toxicol. Lett., 143, 1-7, 2003(PMID: 12697374)., Biochem. Biophys. Res. Commun., 314, 805-809, 2004(PMID: 14741707)., Cancer Lett., 220, 197-210, 2005(PMID: 15766595)., Cancer Res., 66, 535-542, 2006(PMID: 16397270)., Cancer Res., 67, 3945-3954, 2007(PMID: 17440110)., Cell Signal., 19, 185-193, 2007(PMID: 16887331)., Cancer Res., 69, 1392-1399, 2009(PMID: 19190350)., Cancer Res., 70, 6659-6669, 2010(PMID: 20710045).
Comments:
Deposited by:
Restriction:
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
11, 12 11 8, 9 11, 12 14, 15 6 X 9, 12 10
Photo: