細胞株の詳細データ
TKG 0482 ：： KMST-6
| ID | TKG 0482 | ||||||||||||||||||
| Cell name | KMST-6 | ||||||||||||||||||
| Data accepted | |||||||||||||||||||
| Animal | Human. | ||||||||||||||||||
| Scientific name | Homo sapiens | ||||||||||||||||||
| Sex | |||||||||||||||||||
| Case history | |||||||||||||||||||
| Tissue | Embryonal fibroblas. | ||||||||||||||||||
| Genetics | |||||||||||||||||||
| Life span | Infinite | ||||||||||||||||||
| Morphology | Fibroblast-like | ||||||||||||||||||
| Medium | E-MEM + 10% FBS or RPMI 1640 + 10% FBS | ||||||||||||||||||
| Passage method | 0.25% trypsin | ||||||||||||||||||
| Characteristics | A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210 | ||||||||||||||||||
| Misc | Characteristics Embryonal fibroblast immortalized by 60-Co irradiation (400 Rad x 1 time and 200 rad x 12 times). Non-tumorigenic. | ||||||||||||||||||
| CO2 concentration | 5% | ||||||||||||||||||
| Subculture frequency | 1:10, twice a week. | ||||||||||||||||||
| Classification | |||||||||||||||||||
| Mycoplasma | PCR: -, Hoechst DNA staining: - | ||||||||||||||||||
| Established by | Namba, M. | ||||||||||||||||||
| References | Human Cell, 6, 253-259, 1993(PMID: 8148305)., J. Cancer Res. Clin. Oncol., 119, 522-526, 1993(PMID: 8100822)., Mol. Cell. Biol., 13, 6036-6043, 1993(PMID: 8105370)., Human Cell, 7, 88-94, 1994(PMID: 8279474)., In Vitro Cell Dev. Biol. Anim.,, 30A, 199-201, 1994(PMID: 7520808)., Cell Struct. Funct., 20, 41-45, 1995(PMID: 7540958)., Electrophoresis, 16, 1067-1073, 1995(PMID: 7498129)., "Senescence, Immortalization, Transformation." Ed. Namba, Watanabe & Hayflick, Kyoritus Pub. (ISBN4-320-06107-1), 1995. Jap. J. Cancer Res., 86, 35-40, 1995(PMID: 7737907)., Human Genet., 97, 1-6, 1996(PMID: 8557247)., J. Cancer Res. Clinic. Oncol., 122, 249-253, 1996(PMID: 8601579)., In Vitro Cell Dev. Biol. Anim., 34, 463-467, 1998(PMID: 9661049).. This cell line is supplied by Health Science Research Resources Bank (HSRRB). |
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| References by users | Clin. Cancer Res., 11, 6800-6806, 2005(PMID: 16203767), Oncol Rep. 2017 Jul;38(1):31-42(PMID: 28560460), Monoclon Antib Immunodiagn Immunother. 2018 Apr;37(2):110-115(PMID: 29608408), Toxicology. 2021 Apr 15:453:152723(PMID: 33596451). | ||||||||||||||||||
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| Restriction | |||||||||||||||||||
| DNA Profile (STR-PCR) |
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| Photo |  (x100) |