細胞株の詳細データ

TKG 0571 :: A20

ID: TKG 0571
Cell name: A20 (mouse B lymphoma)
Data accepted:
Animal: BALB/c mouse
Scientific name: Mus musculus
Sex:
Case history:
Tissue:
Genetics:
Life span: Infinite.
Morphology: Lymphoblast-like
Medium: RPMI-1640 +10% FBS
Passage method: Dilution
Characteristics: BALB/c lymphoma. Established from spontaneous reticulum cell lymphoma found in an old BALB/c AnN. These cells are IgG(+), Ia(+), Fc-R(+), IgM(-), IgA(-) and complement receptor (-). Diploid and tumorigenic surfaci Ig, however, cells were grown in RPMI-1640 they express a high level of surface Ig. T cell factors and mitogens can induce these cells tosecrete IgG extracellularly. This cell line can present both alloantigens and protein antigens.  A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
CO2 concentration: 5%
Subculture frequency:
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Isozyme analysis: LD
Established by:
References: J. Immunol., 122, 549-554, 1979(PMID: 310843)., J. Exp. Med., 155, 445-459, 1982(PMID: 6460073).
References by users: Immunol. Lett., 98, 232-244, 2005(PMID: 15860223).
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