細胞株の詳細データ

TKG 0593 :: ISO-HAS

ID TKG 0593
Cell name ISO-HAS
Data accepted
Animal Human
Scientific name Homo sapiens
Sex
Case history
Tissue Hemangiosarcoma arising on scalp
Genetics
Life span Infinite
Morphology Polygonal to cobblestone appearance at confluence.
Medium DMEM (high glucose) + 15% FBS + ISOS-1 conditioned medium 50%.*The ISOS-1 conditioned medium was harvested after confluent ISOS-1 cells were cultured with medium for 3 to 4 days and filtered by a 0.22-μm Bottle Top Filter.
Passage method 0.25% Trypsin-EDTA
Characteristics Angiosarcoma. Endothelial cell like. Poorly differentiated. This cell line displays endothelial cell-like phenotype such as Dil-Ac-LDL uptake and UEA-1lectin-binding. Weakly positive for von Willebrand factor. CD31 positive.HLA-ABC positive. HLA-DR negative. A point mutation in p 53 is reported.This cell line was established from 84-year old male. Transplantable to SCIDmice. Restriction: Agreement from Dr. Masuzawa required.
CO2 concentration 5%
Subculture frequency 1:1 ~ 1:2, once or twice / week
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Isozyme analysis LD
Established by Masuzawa, M. (Kitasato Univ. Dermatology)
References Int. J. Cancer, 81, 305-308, 1999.PMID: 10188735
References by users Microvasc Res. 2013 Jul:88:25-31(PMID: 23567873), Cancer Chemother Pharmacol. 2014 Feb;73(2):259-70(PMID: 24253175), J Dermatol Sci. 2015 Apr;78(1):44-50(PMID: 25726712).
Comments
Deposited by Masuzawa, M. (Kitasato Univ. Dermatology)
Restriction
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
10 8 10,11 11 14 7,9 XY 8,11 11
Photo