ID |
TKG 0593 |
Cell name |
ISO-HAS |
Data accepted |
|
Animal |
Human |
Scientific name |
Homo sapiens |
Sex |
|
Case history |
|
Tissue |
Hemangiosarcoma arising on scalp |
Genetics |
|
Life span |
Infinite |
Morphology |
Polygonal to cobblestone appearance at confluence. |
Medium |
DMEM (high glucose) + 15% FBS + ISOS-1 conditioned medium 50%.*The ISOS-1
conditioned medium was harvested after confluent ISOS-1 cells were cultured
with medium for 3 to 4 days and filtered by a 0.22-μm Bottle Top Filter. |
Passage method |
0.25% Trypsin-EDTA |
Characteristics |
Angiosarcoma. Endothelial cell like. Poorly differentiated. This cell line
displays endothelial cell-like phenotype such as Dil-Ac-LDL uptake and
UEA-1lectin-binding. Weakly positive for von Willebrand factor. CD31 positive.HLA-ABC
positive. HLA-DR negative. A point mutation in p 53 is reported.This cell
line was established from 84-year old male. Transplantable to SCIDmice.
Restriction: Agreement from Dr. Masuzawa required. |
CO2 concentration |
5% |
Subculture frequency |
1:1 ~ 1:2, once or twice / week |
Classification |
|
Mycoplasma |
PCR: -, Hoechst DNA staining: - |
Isozyme analysis |
LD |
Established by |
Masuzawa, M. (Kitasato Univ. Dermatology) |
References |
Int. J. Cancer, 81, 305-308, 1999.PMID: 10188735 |
References by users |
Microvasc Res. 2013 Jul:88:25-31(PMID: 23567873), Cancer Chemother Pharmacol. 2014 Feb;73(2):259-70(PMID: 24253175), J Dermatol Sci. 2015 Apr;78(1):44-50(PMID: 25726712). |
Comments |
|
Deposited by |
Masuzawa, M. (Kitasato Univ. Dermatology) |
Restriction |
|
DNA Profile
(STR-PCR) |
D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
10 |
8 |
10,11 |
11 |
14 |
7,9 |
XY |
8,11 |
11 |
|
Photo |
|