ID |
TKG 0595 |
Cell name |
MM46-APR-MUC1 cl.1 |
Data accepted |
|
Animal |
mouse. |
Scientific name |
Mus musculus |
Sex |
|
Case history |
|
Tissue |
Mammary carcinoma |
Genetics |
|
Life span |
Infinite. |
Morphology |
Lymphoblast-like |
Medium |
RPMI-1640 supplemented with 10% FBS, G418 (200 micrigran/ml), Pc and SM. |
Passage method |
Dilution |
Characteristics |
Mouse mammary carcinoma cell line transfected with human MUC1 (APR-MUC1) gene.
Marked MUC1 expression. Transplantable to C3H mouse (i.p.). When inoculated to
SCID mice (i.p., two millionn cells per mouse), they come down 21-24 days after
inoculation. |
CO2 concentration |
5% |
Subculture frequency |
1:5, twice a week. |
Classification |
|
Mycoplasma |
PCR: -, Hoechst DNA staining: - |
Isozyme analysis |
LD |
Established by |
|
References |
See MM46 cell line in this home page. |
References by users |
|
Comments |
|
Deposited by |
Kudo T. (IDAC, Tohoku Univ.) |
Restriction |
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Photo |
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