細胞株の詳細データ

TKG 0596 ：： NSO-SFM

ID：	TKG 0596
Cell name：	NSO-SFM.
Data accepted：
Animal：	BALB/c mouse.
Scientific name：	Mus musculus.
Sex：
Case history：
Tissue：
Genetics：
Life span：	Infinite.
Morphology：	Lymphoblast-like.
Medium：	Serum-free and protein-free hybridoma medium (Hybridoma-SFM medium, Life Technologies) supplemented with HT medium, Penicillin, Streptomycin and NaHCO3. For preparation of 2000 ml medium, 35.8g Hybridoma-SFM (powder, Cat. 23600), NaHCO3 6.5g, 10ml 50X Ht (Cat. 1680949,ICN), 2 ml Penicillin and Streptomycin (1000X) should be dissolved to 2000 ml MilliQ water and filtrated.
Passage method：	Dilution.
Characteristics：	NSO mouse myeloma cell line (TKG 0594) adapted to serum-free culture medium. A non-Ig-secreting, non-light chain-synthesising subclone of NS-2 myeloma. HAT-sensitive.
CO2 concentration：	5%
Subculture frequency：
Classification：
Mycoplasma：	PCR: -, Hoechst DNA staining: -
Isozyme analysis：	LD, NP
Established by：	T. Kudo (IDAC), Tohoku Univiersity.
References：	Methods in Enzymology 73B, 3, 1981.
References by users：
Comments：
Deposited by：
Restriction：
Photo：	Photo(x100)