細胞株の詳細データ

TKG 0596 :: NSO-SFM

ID: TKG 0596
Cell name: NSO-SFM.
Data accepted:
Animal: BALB/c mouse.
Scientific name: Mus musculus.
Sex:
Case history:
Tissue:
Genetics:
Life span: Infinite.
Morphology: Lymphoblast-like.
Medium: Serum-free and protein-free hybridoma medium (Hybridoma-SFM medium, Life Technologies) supplemented with HT medium, Penicillin, Streptomycin and NaHCO3. For preparation of 2000 ml medium, 35.8g Hybridoma-SFM (powder, Cat. 23600), NaHCO3 6.5g, 10ml 50X Ht (Cat. 1680949,ICN), 2 ml Penicillin and Streptomycin (1000X) should be dissolved to 2000 ml MilliQ water and filtrated.
Passage method: Dilution.
Characteristics: NSO mouse myeloma cell line (TKG 0594) adapted to serum-free culture medium. A non-Ig-secreting, non-light chain-synthesising subclone of NS-2 myeloma. HAT-sensitive.
CO2 concentration: 5%
Subculture frequency:
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Isozyme analysis: LD, NP
Established by: T. Kudo (IDAC), Tohoku Univiersity.
References: Methods in Enzymology 73B, 3, 1981.
References by users:
Comments:
Deposited by:
Restriction:
Photo:
 Photo(x100)