細胞株の詳細データ

TKG 0620 :: RB6-8C5

ID TKG 0620
Cell name RB6-8C5.
Data accepted Jan, 22, 2003.
Animal Rat hybridoma.
Scientific name
Sex
Case history
Tissue B hybridoma
Genetics
Life span Infinite
Morphology Lymphoblasto-like.
Medium RPMI-1640 +10% FBS.
Passage method Dilution.
Characteristics Rat hybridoma producing antibody reactive with mouse neutrophil and eosinophil cells. This antibody does not react with mouse lymphocytes and macrophage cells Rat IgG2b. When admisnistered into mouse in vivo, neutrophile and eosinophile cells are removed. Authors who use this antibody should add acknowledgment to Dr. R. Coffman (DNAX research Inst.) and the follwing papers as references; Clin. Exp.Immunol., 120, 125-133, 2000. Eur. J. Immunol., 31, 265-275, 2001.
CO2 concentration 5%
Subculture frequency 1:10 ~ 1:20, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Isozyme analysis
Established by Dr. R. Coffman (DNAX Research Inst. Palo Alto, CA.)
References Infect. Immunol., 61, 5090-5096, 1993(PMID: 8225586)., J. Exp. Med., 179, 259-268, 1994(PMID: 8270870)., J. Immunol., 152, 1836-1846, 1994(PMID: 8120393)., Infect. Immunol., 62, 5161-5163, 1994(PMID: 7927800)., J. leukoc. Biol., 57, 827-831, 1995(PMID: 7790763).
References by users J Virol. 2008 Mar;82(6):2772-83(PMID: 18184718), J. Virol., 88, 7178-7188, 2014 (PMID: 24719427).
Comments In publishing the research results obtained by use this cell line, a citation of the following literatures is requested.
Clin Exp Immunol., 120, 125-133,2000., Eur J Immunol., 31, 265-275, 2001.

Please describe an acknowledgement of Dr. R Coffman (DNAX Research Institute, Palo Alto, CA) in the research results.
Deposited by Dr. Sendo. (Yamagata University)
Restriction
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