細胞株の詳細データ
TKG 0622 :: TBR10-1
ID | TKG 0622 |
Cell name | TBR10-1 |
Data accepted | March 2000. |
Animal | C57BL6 SV40(tsA58) transgenic mouse. |
Scientific name | |
Sex | |
Case history | |
Tissue | Bone marrow. |
Genetics | |
Life span | Infinite |
Morphology | Fibroblast-like |
Medium | RITC80-7, 2% FBS, 10ng/ml EGF, 10ug/ml Tf, & 1ug/ml Insulin. |
Passage method | 0.25% trypsin-EDTA-PBS, 33oC culture. |
Characteristics | Bone marrow fibroblast. Optimal culture temperature 33oC, but culturable at 37oC, using type I collagen 6 well plate (Sumitomo bakelite MS-0006K plate). |
CO2 concentration | 5% |
Subculture frequency | 1:5, twice a week. |
Classification | |
Mycoplasma | PCR: -, Hoechst DNA staining: ± |
Isozyme analysis | LD, NP |
Established by | Dr. Nobuaki Yanai (Institute of DEvel. Aging and Cancer). Anyone wants to use, contact Prof. Obinata. |
References | J Cell Physiol. 1995 Jul;164(1):55-64 PMID: 7790397 |
References by users | |
Comments | |
Deposited by | Dr. Yanai |
Restriction | In publishing the research results obtained by use this cell line, a citation of the following literature is necessary. J Cell Physiol. 1995 Jul;164(1):55-64 PMID: 7790397 |
Photo | Photo(x100) |