細胞株の詳細データ

TKG 0622 :: TBR10-1

ID TKG 0622
Cell name TBR10-1
Data accepted March 2000.
Animal C57BL6 SV40(tsA58) transgenic mouse.
Scientific name
Sex
Case history
Tissue Bone marrow.
Genetics
Life span Infinite
Morphology Fibroblast-like
Medium RITC80-7, 2% FBS, 10ng/ml EGF, 10ug/ml Tf, & 1ug/ml Insulin.
Passage method 0.25% trypsin-EDTA-PBS, 33oC culture.
Characteristics Bone marrow fibroblast. Optimal culture temperature 33oC, but culturable at 37oC, using type I collagen 6 well plate (Sumitomo bakelite MS-0006K plate).
CO2 concentration 5%
Subculture frequency 1:5, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: ±
Isozyme analysis LD, NP
Established by Dr. Nobuaki Yanai (Institute of DEvel. Aging and Cancer). Anyone wants to use, contact Prof. Obinata.
References J Cell Physiol. 1995 Jul;164(1):55-64 PMID: 7790397
References by users
Comments
Deposited by Dr. Yanai
Restriction In publishing the research results obtained by use this cell line, a citation of the following literature is necessary.
J Cell Physiol. 1995 Jul;164(1):55-64 PMID: 7790397
Photo
 Photo(x100)