| ID： |
TKG 0629 |
| Cell name： |
MEC |
| Data accepted： |
|
| Animal： |
Human |
| Scientific name： |
Homo sapiens |
| Sex： |
|
| Case history： |
|
| Tissue： |
Human cholangiocarcinoma
|
| Gentics： |
|
| Life span： |
Infinite |
| Morphology： |
Epithelial-like
|
| Medium： |
RPMI-1640 +10% FBS, penicillin, streptomycin
|
| Passage method： |
0.25% trypsin |
| Characteristics： |
Human choangiocarcinoma cell line established from pleural
fluid of a cholangio-carcinoma patient. CA19-9 positive. Transplantable to nude
mouse. Culturable with serum-free medium (Ham F12). A culture submitted to the
Cell Resource Center for Biomedical Research was found to be contaminated with
mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210. |
| CO2 concentration： |
|
| Subculture frequency： |
|
| Classification： |
|
| Mycoplasma： |
|
| Isozyme analysis： |
|
| Established by： |
Kohichi Yoshida First dept. of Surgery, ST. Marianna
UNiversity School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki 213,
Japan. |
| References： |
Human Cell, 3(4), 346-351, 1990. PMID: 1965684 |
| References by users： |
|
| Comments： |
|
| Deposited by： |
Prof. Kubota, First Department of surgery, St.
Marianna University.
|
| Restriction： |
|
DNA Profile
（STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 11, 13 |
9, 11 |
9.3, 11 |
9, 12 |
17, 18 |
9.3 |
XY |
8, 9 |
10, 14 |
|
| Photo： |
 |
