細胞株の詳細データ

TKG 0661 :: SMAP1dm

ID TKG 0661
Cell name SMAP1dm (SMAP1-deficient macrophage)
Data accepted
Animal SMAP1(-/-) mouse(C57/BL6).
Scientific name Mus musculus.
Sex F.
Case history
Tissue Bone marrow.
Genetics
Life span Infinite.
Morphology
Medium complete RPMI-1640 + 10% FBS+PC/SM
Passage method 0.05% Trypsin + 1mM EDTA
Characteristics Immunophenotype are Mac1+, c-kitlow, CD34low, CD71low, Gr1-, Ter119-, CD3- and B220-. Expresses soluble form of SCF. Differentiated cell line derived from leukemia cell of monocytic leukemia. c-Kit inhibitors (imatinib, ISCK03) decrease cell viability. This cell line is killed by 4μg/ml of puromycin.
CO2 concentration 5%
Subculture frequency 1:5, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Isozyme analysis LD
Established by Kon S. (IDAC, Tohoku Univ.)
References Kon S, Tanabe K, Watanabe T, Sabe H, Satake M. Exp Cell Res., 314(7):1415-1428 (2008).
References by users
Comments
Deposited by Satake M. (IDAC, Tohoku Univ.)
Restriction
Photo
 Photo(x100)