細胞株の詳細データ
TKG 0661 :: SMAP1dm
ID | TKG 0661 |
Cell name | SMAP1dm (SMAP1-deficient macrophage) |
Data accepted | |
Animal | SMAP1(-/-) mouse(C57/BL6). |
Scientific name | Mus musculus. |
Sex | F. |
Case history | |
Tissue | Bone marrow. |
Genetics | |
Life span | Infinite. |
Morphology | |
Medium | complete RPMI-1640 + 10% FBS+PC/SM |
Passage method | 0.05% Trypsin + 1mM EDTA |
Characteristics | Immunophenotype are Mac1+, c-kitlow, CD34low, CD71low, Gr1-, Ter119-, CD3- and B220-. Expresses soluble form of SCF. Differentiated cell line derived from leukemia cell of monocytic leukemia. c-Kit inhibitors (imatinib, ISCK03) decrease cell viability. This cell line is killed by 4μg/ml of puromycin. |
CO2 concentration | 5% |
Subculture frequency | 1:5, twice a week. |
Classification | |
Mycoplasma | PCR: -, Hoechst DNA staining: - |
Isozyme analysis | LD |
Established by | Kon S. (IDAC, Tohoku Univ.) |
References | Kon S, Tanabe K, Watanabe T, Sabe H, Satake M. Exp Cell Res., 314(7):1415-1428 (2008). |
References by users | |
Comments | |
Deposited by | Satake M. (IDAC, Tohoku Univ.) |
Restriction | |
Photo | Photo(x100) |