細胞株の詳細データ

TKG 0666 :: B16 C2W

ID TKG 0666
Cell name B16 C2W
Data accepted
Animal Mouse (C57BL/6J).
Scientific name Mus musculus.
Sex
Case history
Tissue Melanoma.
Genetics
Life span Infinite
Morphology Epithelial-like.
Medium RPMI-1640 + 10% FBS.
Passage method 0.05% Trypsin.
Characteristics Mouse melanoma, melanin production (-).  This tissue culture cell line was established from a cell line maintained by in vivo transplantation. Conditions for transplantation, sc:80-100:20-30 (transplantation site : transplantation rate : survival days).  The cell line was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
CO2 concentration 5%
Subculture frequency 1:4, 2 ~ 3 times / week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Isozyme analysis
Established by Oikawa, A
References Cell differentiation, 1, 149-155, 1972.PMID: 4209534.
References by users Mol Cell Biol. 2019 Aug 12;39(17):e00154-19(PMID: 31208979).
Comments
Deposited by Oikawa, A. (RITC).
Restriction
Photo
 Photo(x100)