細胞株の詳細データ

TKG 0666 :: B16 C2W

ID: TKG 0666
Cell name: B16 C2W
Data accepted:
Animal: Mouse (C57BL/6J).
Scientific name: Mus musculus.
Sex:
Case history:
Tissue: Melanoma.
Genetics:
Life span: Infinite
Morphology: Epithelial-like.
Medium: RPMI-1640 + 10% FBS.
Passage method: 0.05% Trypsin.
Characteristics: Mouse melanoma, melanin production (-).  This tissue culture cell line was established from a cell line maintained by in vivo transplantation. Conditions for transplantation, sc:80-100:20-30 (transplantation site : transplantation rate : survival days).  The cell line was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
CO2 concentration: 5%
Subculture frequency:
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Isozyme analysis:
Established by: Oikawa, A
References: Cell differentiation, 1, 149-155, 1972.PMID: 4209534.
References by users:
Comments:
Deposited by: Oikawa, A. (RITC).
Restriction:
Photo:
 Photo(x100)