細胞株の詳細データ
TKG 0666 :: B16 C2W
ID | TKG 0666 |
Cell name | B16 C2W |
Data accepted | |
Animal | Mouse (C57BL/6J). |
Scientific name | Mus musculus. |
Sex | |
Case history | |
Tissue | Melanoma. |
Genetics | |
Life span | Infinite |
Morphology | Epithelial-like. |
Medium | RPMI-1640 + 10% FBS. |
Passage method | 0.05% Trypsin. |
Characteristics | Mouse melanoma, melanin production (-). This tissue culture cell line was established from a cell line maintained by in vivo transplantation. Conditions for transplantation, sc:80-100:20-30 (transplantation site : transplantation rate : survival days). The cell line was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210. |
CO2 concentration | 5% |
Subculture frequency | 1:4, 2 ~ 3 times / week. |
Classification | |
Mycoplasma | PCR: -, Hoechst DNA staining: - |
Isozyme analysis | |
Established by | Oikawa, A |
References | Cell differentiation, 1, 149-155, 1972.PMID: 4209534. |
References by users | Mol Cell Biol. 2019 Aug 12;39(17):e00154-19(PMID: 31208979). |
Comments | |
Deposited by | Oikawa, A. (RITC). |
Restriction | |
Photo | Photo(x100) |