細胞株の詳細データ
TKG 0713 :: DI8
ID | TKG 0713 |
Cell name | DI8 |
Data accepted | May 18, 2011. |
Animal | cerebroside sulfotransferase deficient mouse |
Scientific name | |
Sex | |
Case history | |
Tissue | Spleen |
Genetics | |
Life span | |
Morphology | Lymphoblast-like |
Medium | For growth and maintenance: RPMI-1640+10% FBS, 1mM sodium Ppyruvate, 2mM L-glutamine, HAT For antibody production: serum-free medium (ASF104, AJINOMOTO)+HAT |
Passage method | Dilution. |
Characteristics | Mouse hybridoma producing anti-sulfatide/seminolipid mouse monoclonal antibody(IgG3). |
CO2 concentration | 5% |
Subculture frequency | 1:1~1:2, twice a week. |
Classification | |
Mycoplasma | PCR: -, Hoechst DNA staining: - |
Established by | Xinyao Cheng (Dept. of Biochemistry, Osaka Univ. Medical School) Koichi Honke (Dept. of Biochemistry, Kochi Univ. Medical School) |
References | J. Biochem. 137:415-421(2005) |
References by users | |
Comments | |
Deposited by | Koichi Honke (Dept. of Biochemistry, Kochi Univ.) |
Restriction | In publishing the research results obtained by use of this cell line, a citation of J. Biochem. 137:415-421(2005) is requested. |
Photo | Photo(x100) |