細胞株の詳細データ

TKG 0713 :: DI8

ID TKG 0713
Cell name DI8
Data accepted May 18, 2011.
Animal cerebroside sulfotransferase deficient mouse
Scientific name
Sex
Case history
Tissue Spleen
Genetics
Life span
Morphology Lymphoblast-like
Medium For growth and maintenance: RPMI-1640+10% FBS, 1mM sodium Ppyruvate,
2mM L-glutamine, HAT
For antibody production: serum-free medium (ASF104, AJINOMOTO)+HAT
Passage method Dilution.
Characteristics Mouse hybridoma producing anti-sulfatide/seminolipid mouse monoclonal antibody(IgG3).
CO2 concentration 5%
Subculture frequency 1:1~1:2, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by Xinyao Cheng (Dept. of Biochemistry, Osaka Univ. Medical School)
Koichi Honke (Dept. of Biochemistry, Kochi Univ. Medical School)
References J. Biochem. 137:415-421(2005)
References by users
Comments
Deposited by Koichi Honke (Dept. of Biochemistry, Kochi Univ.)
Restriction In publishing the research results obtained by use of this cell line, a citation of J. Biochem. 137:415-421(2005) is requested.
Photo
 Photo(x100)