細胞株の詳細データ

TKG 0715 :: ISO-HAS-B

ID TKG 0715
Cell name ISO-HAS-B
Data accepted
Animal Human.
Scientific name Homo sapiens.
Sex M
Case history
Tissue Hemangiosarcoma arising on scalp
Genetics
Life span Infinite
Morphology Polygonal to cobblestone appearance at confluence.
Medium DMEM (high glucose) + 10% FBS.
Passage method 0.25% Trypsin-EDTA
Characteristics
Angiosarcoma. Endothelial cell like. Poorly differentiated. This cell line displays endothelial cell-like phenotype such as Dil-Ac-LDL uptake and UEA-1lectin-binding. Weakly positive for von Willebrand factor. CD31 positive. HLA-ABC positive. HLA-DR negative. A point mutation in p 53 is reported. This cell line is ISO-HAS naturalized to DMEM. Slow-growing.
CO2 concentration 5%
Subculture frequency 1:1, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by Masuzawa M.
(Department of Regulation Biochemistry, Kitasato University School of Allied Sciences)
References
Int. J. Cancer 81: 305-308, 1999. PMID: 10188735, J. Dermatol. Sci. 16: 91-98, 1998. PMID: 9459120
References by users Anticancer Res. 2019 Mar;39(3):1143-1150(PMID: 30842143), Exp Dermatol. 2023 Jan;32(1):50-59(PMID: 36168721).
Comments When you begin to culture this cell line, please be careful not to lower cell density.
1tube --> 60mm cell culture dish x 2 or smaller scale
Deposited by Masuzawa M.
(Department of Regulation Biochemistry, Kitasato University School of Allied Sciences)
Restriction
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
10 8 10,11 11 14 7,9 XY 8, 11 11
Photo