細胞株の詳細データ
TKG 0721 :: Hepa1-3xFlag-AhR-HA-pCEP4(CAG)H12C1
ID | TKG 0721 |
Cell name | Hepa1-3xFlag-AhR-HA-pCEP4(CAG)H12C1 |
Data accepted | March, 2014 |
Animal | Mouse C56BL |
Scientific name | Mus musculus. |
Sex | |
Case history | |
Tissue | Liver |
Genetics | Stable Hepa1c1c7 cl19E4 mutant transfected with 3xFlag-HA-tagged aryl hydrocarbon receptor (AhR) gene. |
Life span | Infinite |
Morphology | Fibroblast-like. |
Medium | DMEM + 5% FBS |
Passage method | 0.25% trypsin/EDTA |
Characteristics | Resistant to 150㎍/ml hygromycin B. Useful for analysis of AhR transcription
factor complex. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210. |
Temperature | 37℃ |
CO2 concentration | 5% |
Subculture frequency | 1:10, twice a week. |
Classification | |
Mycoplasma | PCR: -, Hoechst DNA staining: - |
Established by | Ebina, M. |
References | Ebina M, et al. Biochim. Biophys.Acta,1809,176 (2011) |
References by users | |
Comments | |
Deposited by | Kikuchi, H. |
Restriction | |
Photo |