ID |
TKG 0725 |
Cell name |
Seki |
Data accepted |
December, 2014 |
Animal |
Human. |
Scientific name |
Homo sapiens. |
Sex |
M |
Case history |
|
Tissue |
Ewing's sarcoma. |
Genetics |
Translocation of t(11;22)(q24;q12), EWSR1-Fli1. |
Life span |
Infinite. |
Morphology |
|
Medium |
RPMI1640 + 10% FBS. |
Passage method |
0.05% trypsin/EDTA, collagen coated dish. |
Characteristics |
Transplantable tumor cell line (subcutaneously, Nude mouse, SCID). |
Temperature |
37℃ |
CO2 concentration |
5% |
Subculture frequency |
1:5-1:10, twice a week. |
Classification |
|
Mycoplasma |
PCR: -, Hoechst DNA staining: ± |
Established by |
Nojima, T. |
References |
Igaku no ayumi. 145(7):511-512. Human Cell. 2:192-193(PMID: 2486607). Nagoya Med J. 35:169-180. Genes Chromosome Cancer. 13:126-132(PMID: 7542907). |
References by users |
|
Comments |
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Deposited by |
Nojima, T. |
Restriction |
|
DNA Profile
(STR-PCR) |
D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
10, 12 |
8, 12 |
10, 13 |
12, 13 |
14, 18 |
6, 9 |
XY |
8 |
10, 12 |
|
Photo |
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