細胞株の詳細データ

TKG 0727:: NCB20

ID: TKG 0727
Cell name: NCB20
Data accepted: July, 2015
Animal: Mouse x Chinese hamster
Scientific name: Mus musculus; Cricetulus griseus.
Sex:
Case history:
Tissue: N18TG-2 mouse neuroblastoma x Chinese hamster embryonic brain cells
Genetics:
Life span: Infinite
Morphology:
Medium: DMEM + HAT + 5% FBS
Passage method: 1:5-1:20 with PBS (-) or 0.05% trypsin.
Characteristics: Transplantable tumor cell line. Cholinergic.
Temperature: 37℃
CO2 concentration: 10%
Subculture frequency: Twice a week.
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Species identification by nested PCR:  Only the mouse-specific band was observed. (PMID: 17516125)
Established by: Marshall Nirenberg.
References: Neurochemistry International 61 (2012) 821-827 (PMID: 22414530), Cancer Genetics and Cytogenetics 16, 219-227(1985) (PMID:397859),
Folia phermacol. japon. 77, 99-113 (1981)
References by users:
Comments:
Deposited by: Higashida, H.
(Research Center for Child Mental Development, Kanazawa University)
Restriction:
Photo: