細胞株の詳細データ
TKG 0727:: NCB20
ID | TKG 0727 |
Cell name | NCB20 |
Data accepted | July, 2015 |
Animal | Mouse x Chinese hamster |
Scientific name | Mus musculus; Cricetulus griseus. |
Sex | |
Case history | |
Tissue | N18TG-2 mouse neuroblastoma x Chinese hamster embryonic brain cells |
Genetics | |
Life span | Infinite |
Morphology | |
Medium | DMEM + HAT + 5% FBS |
Passage method | 1:5-1:20 with PBS (-) or 0.05% trypsin. |
Characteristics | Transplantable tumor cell line. Cholinergic. |
Temperature | 37℃ |
CO2 concentration | 10% |
Subculture frequency | Twice a week. |
Classification | |
Mycoplasma | PCR: -, Hoechst DNA staining: - |
Species identification by nested PCR | Only the mouse-specific band was observed. (PMID: 17516125) |
Established by | Marshall Nirenberg. |
References | Neurochemistry International 61 (2012) 821-827 (PMID: 22414530), Cancer Genetics and Cytogenetics 16, 219-227(1985) (PMID:397859), Folia phermacol. japon. 77, 99-113 (1981) |
References by users | |
Comments | |
Deposited by | Higashida, H. (Research Center for Child Mental Development, Kanazawa University) |
Restriction | |
Photo |