細胞株の詳細データ

TKG 0727:: NCB20

ID TKG 0727
Cell name NCB20
Data accepted July, 2015
Animal Mouse x Chinese hamster
Scientific name Mus musculus; Cricetulus griseus.
Sex
Case history
Tissue N18TG-2 mouse neuroblastoma x Chinese hamster embryonic brain cells
Genetics
Life span Infinite
Morphology
Medium DMEM + HAT + 5% FBS
Passage method 1:5-1:20 with PBS (-) or 0.05% trypsin.
Characteristics Transplantable tumor cell line. Cholinergic.
Temperature 37℃
CO2 concentration 10%
Subculture frequency Twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Species identification by nested PCR Only the mouse-specific band was observed. (PMID: 17516125)
Established by Marshall Nirenberg.
References Neurochemistry International 61 (2012) 821-827 (PMID: 22414530), Cancer Genetics and Cytogenetics 16, 219-227(1985) (PMID:397859),
Folia phermacol. japon. 77, 99-113 (1981)
References by users
Comments
Deposited by Higashida, H.
(Research Center for Child Mental Development, Kanazawa University)
Restriction
Photo