細胞株の詳細データ

TKG 0728 :: H6-3C4

ID TKG 0728
Cell name H6-3C4
Data accepted October, 2009.
Animal Human x Mouse
Scientific name
Sex Female
Case history
Tissue Peripheral blood lymphocyte.
Genetics
Life span
Morphology Lymphoblast-like
Medium RPMI-1640+10% FBS, L-glutamine.
Passage method Dilute 1/2 ~ 1/10.
Characteristics Human-mouse hetero hybridoma producing human monoclonal antibody, IgM (λ), with strong sperm immobilizing and sperm agglutinating activities.
Ampicillin resistance.
A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
CO2 concentration 5%
Subculture frequency
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by Shinzo, I
References Jurnal of Reproductive Immunology, 10 (1987) 67-78. PMID: 3295232
References by users
Comments
Deposited by Shinji Komori (Dept. of Obsterics and Gynecology, Hyogo College of Medicine.)
Restriction B: A prior written permission of the register is necessary.
Photo (x100)