細胞株の詳細データ

TKG 0731 :: R-3

ID: TKG 0731
Cell name: R-3
Data accepted: Sept, 2017.
Animal: Rat (Donryu).
Scientific name: Rattus norvegicus.
Sex: M
Case history:
Tissue: Lymph node with metastasis from esophageal tumor.
Genetics:
Life span: Infinite.
Morphology: Squamous cell carcinoma.
Medium: Eagle MEM + 10% FBS.
Passage method: 0.25% trypsin.
Characteristics: This cell line was established from squamous cell carcinoma of the esophagus induced in Donryu rats by N-amyl-N-Methyl-nitrosamine. Transplantable tumor cell line (nude mouse, sc).
A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by treatments with BM cyclin and MC210.
Temperature: 37℃
CO2 concentration: 5%
Subculture frequency: 1:10, twice/week.
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Established by: Iizuka, T and Ichimura, S.
References: Jpn. J. Clin. Oncol. 1981, 11 (3), 487-494.
References by users:
Comments:
Deposited by: Nishida, C. (Genome Dynamics Research Center, Faculty of Science, Hokkaido University.)
Restriction:
Photo: (x100)