細胞株の詳細データ

TKG 0731 :: R-3

ID TKG 0731
Cell name R-3
Data accepted Sept, 2017.
Animal Rat (Donryu).
Scientific name Rattus norvegicus.
Sex M
Case history
Tissue Lymph node with metastasis from esophageal tumor.
Genetics
Life span Infinite.
Morphology Squamous cell carcinoma.
Medium Eagle MEM + 10% FBS.
Passage method 0.25% trypsin.
Characteristics This cell line was established from squamous cell carcinoma of the esophagus induced in Donryu rats by N-amyl-N-Methyl-nitrosamine. Transplantable tumor cell line (nude mouse, sc).
A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by treatments with BM cyclin and MC210.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:10, twice/week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by Iizuka, T and Ichimura, S.
References Jpn. J. Clin. Oncol. 1981, 11 (3), 487-494.
References by users
Comments
Deposited by Nishida, C. (Genome Dynamics Research Center, Faculty of Science, Hokkaido University.)
Restriction
Photo (x100)