細胞株の詳細データ
TKG 0731 :: R-3
ID | TKG 0731 |
Cell name | R-3 |
Data accepted | Sept, 2017. |
Animal | Rat (Donryu). |
Scientific name | Rattus norvegicus. |
Sex | M |
Case history | |
Tissue | Lymph node with metastasis from esophageal tumor. |
Genetics | |
Life span | Infinite. |
Morphology | Squamous cell carcinoma. |
Medium | Eagle MEM + 10% FBS. |
Passage method | 0.25% trypsin. |
Characteristics | This cell line was established from squamous cell carcinoma of the esophagus
induced in Donryu rats by N-amyl-N-Methyl-nitrosamine. Transplantable tumor
cell line (nude mouse, sc). A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by treatments with BM cyclin and MC210. |
Temperature | 37℃ |
CO2 concentration | 5% |
Subculture frequency | 1:10, twice/week. |
Classification | |
Mycoplasma | PCR: -, Hoechst DNA staining: - |
Established by | Iizuka, T and Ichimura, S. |
References | Jpn. J. Clin. Oncol. 1981, 11 (3), 487-494. |
References by users | |
Comments | |
Deposited by | Nishida, C. (Genome Dynamics Research Center, Faculty of Science, Hokkaido University.) |
Restriction | |
Photo | (x100) |