細胞株の詳細データ

TKG 0733 :: HC-4

ID: TKG 0733
Cell name: HC-4
Data accepted: September, 2017
Animal: Human.
Scientific name: Homo sapiens.
Sex:
Case history:
Tissue: Liver.
Genetics:
Life span: Infinite.
Morphology:
Medium: Eagle MEM + 10% FBS
Passage method: 0.25% trypsin.
Characteristics: This cell line was established from hepatocarcinoma of 45-year-old male. Transplantable into nude mice.
A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by treatments with BM cyclin and MC210.
Temperature: 37℃
CO2 concentration: 5%
Subculture frequency: 1:4, twice/week.
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Established by:
References: Mutation Res 1983 109:83-90. PMID: 6300668
References by users:
Comments:
Deposited by: Nishida, C. (Genome Dynamics Research Center, Faculty of Science, Hokkaido University.)
Restriction:
DNA Profile
(STR-PCR)
:
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
10 11 10, 12 9 14, 18 6, 9 X 9 13
Photo: (x100)