細胞株の詳細データ
TKG 0750:: Replic
ID | TKG 0750 |
Cell name | Replic |
Data accepted | August, 2019. |
Animal | Mouse, C57BL/6 |
Scientific name | Mus musculus. |
Sex | Male. |
Case history | |
Tissue | Kidney, REP (renal erythropoietin-producing) cells. |
Genetics | |
Life span | Infinite. |
Morphology | Fibroblast-like |
Medium | DMEM high glucose+ 10% FBS |
Passage method | Accutase (Nakarai 12679-54) or 0.05% trypsin. |
Characteristics | The immortalized cell line prepared by the exogenous expression of oncogenic
H-RAS (G12V). tdTomato-positive. High-level TGFβ expression. This cell line was isolated from Epo(GFP/wt)Tg(3.3kEpo)Tg(EpoCre)Rosa26(LSLtdTomato) genotype mice. A culture submitted to Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by treatments with BM Cyclin and MC210. |
Temperature | 37℃ |
CO2 concentration | 5% |
Subculture frequency | 1:5 ~ 1:10, twicw a week |
Classification | |
Mycoplasma | PCR: -, Hoechst DNA staining: - |
Established by | Suzuki, N. |
References | Sci Rep 9(1):11254(2019). PMID: 31375751. |
References by users | |
Comments | |
Deposited by | Suzuki,N. |
Restriction | C: A prior written permission of the originator is necessary. |
Photo |