ID |
TKG 0757 |
Cell name |
mTX9 |
Data accepted |
January, 2019. |
Animal |
Mouse x Rat
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Scientific name |
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Sex |
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Case history |
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Tissue |
B cell hybridoma. |
Genetics |
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Life span |
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Morphology |
Lymphoblast-like. |
Medium |
RPMI1640 + 10% FBS |
Passage method |
Dilution. |
Characteristics |
Immune animal is Wistar rat. Antigen is mouse testicular homogenate named
TEX101. Mouse-Rat hybridoma producing rat monoclonal antibody, IgG2a, kappa.
HAT resistance. This cell line can be used for immunoprecipitation, flow
cytometry and non-reducing western blotting. It may also be used for immunohistochemical
staining. Transplantation into C.B-17/Icr-scid/scid mice induces ascites.
|
Temperature |
37℃ |
CO2 concentration |
5% |
Subculture frequency |
1:4, twice a week. |
Classification |
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Mycoplasma |
PCR: -, Hoechst DNA staining: - |
Established by |
Tsukamoto, H. |
References |
Sci Rep.2016 6:23616 (PMID: 270005865) |
References by users |
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Comments |
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Deposited by |
Tsukamoto, H. |
Restriction |
C: A prior written permission of the originator is necessary. |
Photo |
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