細胞株の詳細データ
TKG 0764:: 1B12
ID | TKG 0764 |
Cell name | 1B12 |
Data accepted | January, 2019 |
Animal | Mouse x Rat |
Scientific name | |
Sex | |
Case history | |
Tissue | B cell hybridoma. |
Genetics | |
Life span | |
Morphology | Lymphoblast-like. |
Medium | RPMI1640 + 10% FBS |
Passage method | Dilution. |
Characteristics | Wistar rats were immunized with human CD14-expressing NRK-52E transfected
cells. Mouse - Rat hybridoma producing rat monoclonal antibody, IgG2a,
kappa. It doesn't cross-react with mouse CD14. HAT resistance. This cell line can be used for immunoprecipitation and flow cytometry. Transplantation into C.B-17/Icr-scid/scid mice induces ascites. |
Temperature | 37℃ |
CO2 concentration | 5% |
Subculture frequency | 1:1 ~ 1:3, twice a week. |
Classification | |
Mycoplasma | PCR: -, Hoechst DNA staining: - |
Established by | Tsukamoto, H. |
References | J Immunol. 2013 190(1):195-204 (PMID: 23203928). |
References by users | |
Comments | |
Deposited by | Tsukamoto, H. |
Restriction | C: A prior written permission of the originator is necessary. |
Photo |