細胞株の詳細データ
TKG 0766:: GFP2
ID | TKG 0766 |
Cell name | GFP2 |
Data accepted | January, 2019. |
Animal | Mouse x Rat |
Scientific name | |
Sex | |
Case history | |
Tissue | B cell hybridoma. |
Genetics | |
Life span | |
Morphology | Lymphoblast-like. |
Medium | RPMI1640 + 10% FBS |
Passage method | Dilution. |
Characteristics | Wistar rats were immunized in the foot pads with EGFP. Mouse-Rat hybridoma
producing rat monoclonal antibody, IgG2a, kappa. HAT resistance. This cell line can be used for immunoprecipitation for antigen with EGFP in C-terminal and FACS. Transplantation into C.B-17/Icr-scid/scid mice induces ascites. |
Temperature | 37℃ |
CO2 concentration | 5% |
Subculture frequency | 1:4 ~ 1:10, twice a week. |
Classification | |
Mycoplasma | PCR: -, Hoechst DNA staining: - |
Established by | Tsukamoto, H. |
References | J Biol Chem.2018 293(26):10186-10201.(PMID: 29760187) |
References by users | |
Comments | |
Deposited by | Tsukamoto, H. |
Restriction | C: A prior written permission of the originator is necessary. |
Photo | (x100) (x200) |